TL series tissue ball mills have a pair of high speed/amplitude arms, using irregular friction and impact of beads in tubes, finish sample preparation in seconds to minutes. Suitable for grind, crush, homogenizing for plant, animal and cells, at a smallest size of 5um.

For different material, pick different kinds of beads, speed and time. Here are some animal sample protocols for record.

Sample 1, insect sample

User: Southwest University

Sample: silkworm moth, silkworm cocoon.

1.       Cut moth body and wings into pieces at size of 8mm; put them into 2ml tube.

2.       Add one 5mm carbide tungsten beads

3.       Load tubes onto adapter rack, add liquid nitrogen in tube to freeze for 3~5mins. Close tube lids; drown the adapter in liquid nitrogen for another 3mins.

4.       Immediately fix the adapter onto shaking arm.

5.       Set speed to 1600, time to 2mins. Samples are fully destructed to fine powder, ready to extract DNA. Same protocol can be applied to cocoon.

This protocol is also available for other insect species nucleic acid and protein extract and analysis. 

Sample 2 mammal organs

Place: Southwest University

Sample: rat heart, spleen, liver for DNA extraction.

1.       Take -80℃preserved rat organ at size of no larger than 5mm, put into 2ml round tipped tube.

2.       Add Protease Inhibitor, defreeze for 3~5mins.

3.       Add1-2 5mm steel beads.

4.       Set speed at 1500rpm, time at 2min.

All three kinds of organ are basically destructed, reached the requirement for DNA extraction (verified in later experiment). Liver sample is fully destructed, while after first grind, visible fragment can be found in spleen and heart sample, and this can be improved by repeat grind cycles (fresh tissue does not happen with this issue).

This protocol is also available for other mammal organs for high number of low mass samples, for preparation of molecular studies.

TL series tissue ball mills are suitable for many types of animal organs; we will continue to test at users demand. With types of adapter and jars, TL series is flexible for various experiment requirements in molecular biology fields, such as biomedical, agriculture, food, custom inspection and detection.